The P2X7 Receptor: Methods and Protocols by Annette Nicke Paperback Book

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Seller: the_nile_uk_store ✉️ (20,008) 98.3%, Location: London, GB, Ships to: WORLDWIDE, Item: 364788937235 The P2X7 Receptor: Methods and Protocols by Annette Nicke Paperback Book. How Structural Biology Has Directly Impacted Our Understanding of P2X Receptor Function and Gating Steven E. Mansoor 2. Preparation of the Murine Anti-Human P2X7 Receptor Monoclonal Antibody (Clone L4) Amal Elhage, Ross J. Turner, Peter Cuthbertson, Debbie Watson, and Ronald Sluyter 6. The Nile on eBay   FREE SHIPPING UK WIDE  

The P2X7 Receptor

by Annette Nicke

This detailed volume covers diverse aspects of P2X7 receptor analysis, ranging from its molecular structure to related pharmacological and immunological tools, via its analysis in heterologous expression systems as well as assays using primary cells and whole animal models.

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Publisher Description

This detailed volume covers diverse aspects of P2X7 receptor analysis, ranging from its molecular structure to related pharmacological and immunological tools, via its analysis in heterologous expression systems as well as assays using primary cells and whole animal models. After three introductory chapters that focus on its structure, ligands, and physiological functions, the book details the generation of antibody and nanobody tools for P2X7 receptors, provides protocols for the analysis of expressed P2X7 receptors with a focus on their electrophysiological analysis, as well as protocols for the investigation of P2X7 down-stream signaling in immune cells by flow cytometry. Mouse models and procedures suited to investigate P2X7-mediated effects in other primary cells and in vivo are also explained. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, The P2X7 Receptor: Methods and Protocols is a valuable reference not only for the growing community fascinated by this unusual ion channel but also for a broad readership interested in ion channels or purinergic receptors.

Table of Contents

1. How Structural Biology Has Directly Impacted Our Understanding of P2X Receptor Function and Gating            Steven E. Mansoor 2. Agonists, Antagonists, and Modulators of P2X7 Receptors            Christa E. Müller and Vigneshwaran Namasivayam 3. Modulation of Cell Energy Metabolism by the P2X7 Receptor            Francesco Di Virgilio, Simonetta Falzoni, Alba Clara Sarti, Paola Chiozzi, Valentina Vultaggio-Poma, and Anna Lisa Giuliani 4. Fully Flexible Ligand Docking for the P2X7 Receptor Using ROSIE            Sudad Dayl and Ralf Schmid 5. Preparation of the Murine Anti-Human P2X7 Receptor Monoclonal Antibody (Clone L4)            Amal Elhage, Ross J. Turner, Peter Cuthbertson, Debbie Watson, and Ronald Sluyter 6. Development of Antibody and Nanobody Tools for P2X7            Tobias Stähler, Welbeck Danquah, Melanie Demeules, Henri Gondé, Romain Hardet, Friedrich Haag, Sahil Adriouch, Friedrich Koch-Nolte, and Stephan Menzel 7. Enhanced Transduction of P2X7-Expressing Cells with Recombinant rAAV Vectors            Anna Marei Mann, Waldemar Schäfer, Sahil Adriouch, Kathleen Börner, Dirk Grimm, Ingke Braren, and Friedrich Koch-Nolte 8. Immunofluorescence Staining of P2X7 Receptors in Whole-Mount Myenteric Plexus Preparations            Tina Jooss, Birgit Kuch, Jiong Zhang, Michael Schemann, and Annette Nicke 9. Established Protocols for cRNA Expression and Voltage-Clamp Characterization of the P2X7 Receptor in Xenopus laevis Oocytes            Günther Schmalzing and Fritz Markwardt 10. A Simplified Protocol to Incorporate the Fluorescent Unnatural Amino Acid ANAP into Xenopus laevis Oocyte-Expressed P2X7 Receptors            Anna Durner and Annette Nicke 11. Using Whole-Cell Electrophysiology and Patch Clamp Photometry to Characterize P2X7 Receptor Currents            Xin Liang, Laura Janks, and Terrance M. Egan 12. Using Symmetrical Organic Cation Solutions to Study P2X7 Ion Permeation            Kate Dunning, Laurie Peverini, and Thomas Grutter 13. Methods for Studying Cholesterol-Dependent Regulation of P2X7 Receptors            Ruth D. Murrell-Lagnado and Toshimitsu Kawate 14. Visualizing P2X7-Dependent Inflammasome Formation in Human Monocytes by Fluorescence Microscopy and Flow Cytometry            Samantha Wittke, Leif Ancker, Sana Javed, and Friedrich Haag 15. Characterization of P2X7 Receptors in Human Blood Cells            Laura Hurtado-Navarro, Alberto Baroja-Mazo, and Pablo Pelegrín 16. Real-Time Flow Cytometry as a Tool to Monitor Cellular Consequences of P2X7 Activation in Multiple Cell Populations Mixed in a Single FACS Tube            Alexander W. Veltkamp, Tim Magnus, and Björn Rissiek 17. Administration of P2X7 Receptor Blockers in Oncological Experimental Models            Elena De Marchi, Anna Pegoraro, and Elena Adinolfi 18. Humanized Mouse Model to Study the P2X7 Receptor in Graft-Versus-Host Disease            Debbie Watson, Sam R. Adhikary, Peter Cuthbertson, Nicholas J. Geraghty, Katrina M. Bird, Amal Elhage, Chloe Sligar, and Ronald Sluyter 19. Analysis of P2X7-Induced Neuronal Branching            Paula Mut-Arbona and Beáta Sperlágh 20. Studying the Role of P2X7 Receptor in Axonal Growth Using In Utero Electroporation Technique            Álvaro Sebastián-Serrano, Carolina Bianchi, Caterina Di Lauro, Lucia Soria-Tobar, Beatriz Álvarez-Castelao, and Miguel Díaz-Hernández 21. Analyzing the Role of the P2X7 Receptor in Epilepsy            Mariana Alves, Laura de Diego-Garcia, and Tobias Engel

Details ISBN1071623869 Author Annette Nicke Pages 394 Publisher Springer-Verlag New York Inc. Series Methods in Molecular Biology Year 2023 Edition 1st ISBN-13 9781071623862 Format Paperback Imprint Springer-Verlag New York Inc. Subtitle Methods and Protocols Place of Publication New York, NY Country of Publication United States Edited by Annette Nicke Illustrations 81 Illustrations, color; 12 Illustrations, black and white; XIV, 394 p. 93 illus., 81 illus. in color. AU Release Date 2023-07-16 NZ Release Date 2023-07-16 UK Release Date 2023-07-16 Edition Description 1st ed. 2022 Alternative 9781071623831 DEWEY 547.596 Series Number 2510 Audience Professional & Vocational Publication Date 2023-07-03 US Release Date 2023-07-03

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  • Condition: New
  • Format: Paperback
  • ISBN-13: 9781071623862
  • Author: Annette Nicke
  • Type: NA
  • Book Title: The P2X7 Receptor
  • Publication Name: NA
  • ISBN: 9781071623862

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